CRISPR–Cas9 has emerged as a powerful technology that enables ready modification of the mammalian genome. The ability to modulate Cas9 activity can reduce off-target cleavage and facilitate precise genome engineering.
Here we report the development of a Cas9 variant whose activity can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT) by fusing the Cas9 enzyme with the hormone-binding domain of the estrogen receptor (ERT2). The final optimized variant, termed iCas, showed low endonuclease activity without 4-HT but high editing efficiency at multiple loci with the chemical. We also tuned the duration and concentration of 4-HT treatment to reduce off-target genome modification. Additionally, we benchmarked iCas against other chemical-inducible methods and found that it had the fastest on rate and that its activity could be toggled on and off repeatedly. Collectively, these results highlight the utility of iCas for rapid and reversible control of genome-editing function.
Cat girls are just normal humans with mental retardation, non developed bodies and typical borderline behavior without being whores, the real problem are ears and tail, but according to manga they are just abused little girls living in a fucked up world with point ears coming from the upper part of their heads and long and thin tails, the first case needs just need some training untill they are legal, then some surgery for ears and tail, the second go to Clinton's island.
Hudson Powell
I'll take the super human package, and if you could throw in a cat-girl, that would be great
Robert Jones
the super human package makes you dislike catgirls because of your super human heteronormative sexuality
Sebastian Williams
are u implying teh gay?
Grayson Baker
so when can we genetically engineer cats that can feed themselves?
Blake Ross
crispies?
Evan Gonzalez
wew
Jackson Sanchez
One life extension package please
Kevin Reed
coming right up
Leo Fisher
A perfect world
John James
Are you ready to receive the Emperor's gene seed?
Adrian Cook
wew
Christopher Williams
TRUMP
Xavier Nelson
...
Luis Price
wew
Gabriel Parker
trump
Oliver Clark
Soon furries will have the power to make furies irl.
Colton Johnson
doubtful
Samuel Bell
crispy?
Benjamin Jenkins
They have the money to afford top-tier artists They will pool together money if it means meet/fuck sessions with their local "pet"
Thomas Cook
do you speak for yourself?
Cooper Evans
So how many more years before I could make myself have 2 extra functional arms, or at least 2 more functional thumbs on each hand? Let's say even if it was expensive and painful, how soon?